Fig. 4: Role of antagomiR-193b-3p in IMQ-induced psoriasis mouse model.

A Expression levels of miR-193b-3p in mouse back skin of different groups. Vehicle means mice injected with antagomiR-NC and treated with Vaseline, antagomiR-NC + IMQ and antagomiR-193 + IMQ means mice injected with antagomiR-NC and antagomiR-193b-3p, respectively, and then treated with IMQ for 5 consecutive days. miR-193b-3p levels were analyzed using qRT-PCR. U6 was used as the internal control. Each bar represents mean ± SEM (n = 8). ^***P < 0.001, compared with the indicated controls. B Representative pictures showing the mouse back skin lesions injectied withantagomiR-193/antagomiR-NC in the presence or obsence of IMQ treatment for 5 consecutive days. C PASI scores of mouse back skin lesions in different groups at different time points. PASI scores of mouse back skin lesions were recorded and analyzed daily. Each bar represents mean ± SEM (n = 8). ^***P < 0.001, compared with the indicated controls. D Representative images showing histological feature of skin tissues derived from mouse back injected with antagomiR-193 or feature of skin tissues derived from mouse back injected with antagomiR-193 or antagomiR-NC in the presence or absence of IMQ treatment for 5 consecutive days. Scar bar of upper panel: 100 µm, scar bar of lower panel: 100 µm. E Quantification data of skin epidermal thickness (upper panel, n = 8) and Ki67-positive cells (lower panel, n = 8) in skin tissues derived from mouse back injected with antagomiR-193 or antagomiR-NC in the presence or absence of IMQ treatment for 5 consecutive days. ^***P < 0.001, compared with the indicated controls. F Representative blots showing the levels of phospho-STAT3 (Tyr705), phospho-STAT3 (Ser727), phospho-NF-κB p65 (Ser311), IL-1β, etc. in skin tissues derived from mouse back injected with antagomiR-193 or antagomiR-NC in the presence or absence of IMQ treatment for 5 consecutive days. Tissues were collected at day 6 and protein levels were detected by western blotting. GAPDH was used as a protein loading control. G mRNA levels of psoriasis-related genes and inflammatory genes in skin tissues derived from mouse back injected with antagomiR-193 or antagomiR-NC in the presence or absence of IMQ treatment for 5 consecutive days. Skin tissues were collected at day 6 and mRNA levels were analyzed using qRT-PCR. GAPDH was used as the internal control. Each bar represents mean ± SEM (n = 8 for each group). ^***P < 0.001, ^**P < 0.01, ^*P < 0.05 compared with the indicated controls.