Fig. 8: ERBB4 rescues the inhibitory effects of miR-193b-3p on keratinocytes. | Cell Death & Disease

Fig. 8: ERBB4 rescues the inhibitory effects of miR-193b-3p on keratinocytes.

From: MiR-193b-3p–ERBB4 axis regulates psoriasis pathogenesis via modulating cellular proliferation and inflammatory-mediator production of keratinocytes

Fig. 8

A The mRNA levels of psoriasis-related genes and inflammatory genes in HaCaT cells co-transfected with miR-193b-3p mimics and ERBB4-overexpression vector (ERBB4OV). Cells were collected 2 days after co-transfection and mRNA levels were analyzed using qRT-PCR. GAPDH was used as the internal control. Each bar represents mean ± SEM (n = 3). ***P < 0.001, **P < 0.01, *P < 0.05, compared with the indicated controls. B Relative proliferation rate of HaCaT cells co-transfected with miR-193/miR-NC and ERBB4OV/CTL. Cell proliferation was analyzed using MTT assay. Each bar represents mean ± SEM (n = 3). *P < 0.05, compared with the indicated controls. C Representative blots showing the levels of phospho-STAT3 (Tyr705), phospho-STAT3 (Ser727), phospho-NF-κB p65 (Ser311), and IL-1β in the keratinocytes co-transfected with miR-193/miR-NC and ERBB4OV/CTL. Protein levels were detected by western blotting. GAPDH was used as a protein loading control.

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