Fig. 5: The effect of vezatin knockdown on the expression levels of GluA1, GluA2, pGluA1-S845, and pGluA1-S831. | Cell Death & Disease

Fig. 5: The effect of vezatin knockdown on the expression levels of GluA1, GluA2, pGluA1-S845, and pGluA1-S831.

From: Vezatin regulates seizures by controlling AMPAR-mediated synaptic activity

Fig. 5

In the PILO-induced epilepsy model: a representative images of western blots showing surface GluA1, total GluA1, surface GluA2, total GluA2, pGluA1-S845, and pGluA1-S831 levels in the hippocampus in the Scr-seq group and shRNA group and b the corresponding statistical analyses (n = 7 per group; surface GluA1, P < 0.001; pGluA1-S845, P < 0.001). In the Mg2+-free solution-induced in vitro seizure-like model, c representative images of immunofluorescence staining showing that surface GluA1 (purple) was localized in Tubb3 (red)-positive and LV-infected neuronal dendrites (green) and (d) the corresponding statistical analysis of the fluorescence intensity of surface GluA1 in the Scr-seq group and shRNA group (P < 0.001). e Representative images of immunofluorescence staining showing that total GluA1 (blue) was localized in Tubb3 (red)-positive and LV-infected (green) neuronal dendrites and (f) the corresponding statistical analysis of the fluorescence intensity of total GluA1 expression in the Scr-seq group and shRNA group (n = 5 independent hippocampal neuron cultures from ten mice per group). Student’s t test; *P < 0.05, **P < 0.01, and ***P < 0.001.

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