Fig. 6: SNHG17 increases c-Myc level and promotes G1/S transition via interacting with LRPPRC. | Cell Death & Disease

Fig. 6: SNHG17 increases c-Myc level and promotes G1/S transition via interacting with LRPPRC.

From: LncRNA SNHG17 interacts with LRPPRC to stabilize c-Myc protein and promote G1/S transition and cell proliferation

Fig. 6

A SNHG17 was associated with the 1035–1369-aa domain of LRPPRC. Biotin-labeled SNHG17 was incubated with GST-tagged full-length or truncated LRPPRC, then pulled down by streptavidin-beads, and the retrieved proteins were detected by Western blotting. B The 1–150-nt fragment of SNHG17 bound to the 1035–1369-aa domain of LRPPRC. Biotin-labeled full-length or truncated SNHG17 was incubated with the GST-tagged 1035–1369-aa of LRPPRC, then pulled down by streptavidin-beads, and the retrieved proteins were detected by Western blotting. C–D Deletion in the 1–150-nt of SNHG17 attenuated the roles of SNHG17 in increasing c-Myc protein level, DNA-replicating cells, and cell growth. Cells stably expressing SNHG17 or 1–150-nt-deleted mutant (SNHG17-Δcore) and the control cells (Ctrl) were subjected to Western blotting (C), EdU incorporation assays (D, left panel), or cell counting (D, right panel). E–F Silencing LRPPRC abrogated the roles of SNHG17 in increasing c-Myc level, DNA-replicating cells, and cell growth. Cells stably expressing SNHG17 and the control cells (Ctrl) were transfected with NC or siLRPPRC, then subjected to Western blotting (E), EdU incorporation assays (F, left panel) or cell counting (F, right panel). SE, short exposure; LE, long exposure. For D and F, error bars represent mean ± SEM from three independent experiments. P values were assessed by unpaired Student’s t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant.

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