Fig. 5: In vivo, targeting Src requires p27-expression to significantly increase CRC Palbo-sensitivity.
A, B Radar (A) and dot (B) plots reporting the number and the distribution of metastatic organs in mice, as assessed by histological analysis. Mice were intracecally injected with wild-type and p27KO HCT-116 cells. Once tumor onset was established, mice were randomly subdivided and treated 5 days/week with vehicle, Palbo or Saracatinib, alone or in combination (combo), as indicated. C, Dot plot reports tumor staging of CRC in mice evaluated according to [42], indicating the tumor spreading from the bowel (I Stage) to the peritoneum (II Stage), to mesenteric lymph node or pancreatic foci (III Stage) and the involvement of intrahepatic or lung metastasis (IV Stage). Each dot represents a different mouse from the indicated groups. D, Representative images of H&E analysis of liver, showing the metastatic involvement of the organ for each mice group, as indicated.
