Fig. 2: Lysosome clusters associate with autophagosomes.
From: Prefused lysosomes cluster on autophagosomes regulated by VAMP8
a Co-localization of autophagosomes and lysosomes in CCCP-treated HeLa cells was observed by SIM. Autophagosomes and lysosomes were stained with 100 nM DAPGreen and 200 nM LTR, respectively, for 30 min to allow the investigation of lysosome–autophagosome association. b 3D SIM image of the enlarged region indicated by white rectangles in a. c Representative 2D SIM images of several lysosomes associated with one autophagosome in each CCCP-treated HeLa cell. d Representative events of lysosomes unassociated, in contact, and fused with the autophagosomes. e Percentage of lysosomes unassociated, in contact, and fused with autophagosomes using the definition described in the text. f Western blot for detecting FIP200 and ATG13 protein expression in CRISPR-Cas9 gene-edited cells. g Lysosome puncta distribution in ATG13KO and FIP200KO HeLa cells with or without CCCP treatment. h The lysosome cluster ratio in WT, ATG13KO, and FIP200KO HeLa cells. Data in e and h are presented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.
