Fig. 6: Zyxin deficiency affects VASP localization.
From: Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression
A, B Platelets were allowed to spread on fibrinogen in the presence of thrombin. Representative confocal images of spread WT platelets stained for VASP (green) and zyxin (red) (A). Representative confocal images of spread WT and Zyx−/− platelets stained for and VASP (red) and F-actin (green) (B). Scale bar: 5 μm. C Representative confocal images of WT and Zyx−/− FL HPC-derived MKs allowed to spread on type I collagen and fibrinogen and stained for VASP (red), F-actin (green), and nuclei (blue). Scale bar: 10 μm. The original magnification of all the images is ×630. Results are representative of five independent experiments. D Filamin A and GPIbα were co-immunoprecipitated with VASP by anti-VASP antibody in WT platelet lysate. Results are representative of five independent experiments.
