Fig. 3: miR-4523 overexpression attenuates DEX-induced oxidative injury in human osteoblasts. | Cell Death & Disease

Fig. 3: miR-4523 overexpression attenuates DEX-induced oxidative injury in human osteoblasts.

From: Phosphoglycerate kinase 1 silencing by a novel microRNA microRNA-4523 protects human osteoblasts from dexamethasone through activation of Nrf2 signaling cascade

Fig. 3

Primary human osteoblasts (A–F) or hFOB1.19 osteoblastic cells (G–J), stably expressing a lentiviral construct encoding the pre-miR-4523 (“lv-pre-miR-4523”) or control miR construct (lv-miRC), were established. Cells were then treated with dexamethasone (DEX, 1 μM) or vehicle control (“Veh”) and cultured for applied time periods. Cellular ROS contents (A, B, and G), lipid peroxidation intensity (C and H), mitochondrial depolarization (D, E, and I), and single-strand DNA contents (ELISA OD, F and J) were tested by the assays mentioned in the text. Data were presented as mean ± standard deviation (SD, n = 5). *P < 0.05 versus “Veh” treatment in “lv-miRC” cells; #P < 0.05 versus “DEX” treatment in “lv-miRC” cells. The experiments were repeated five times with similar results obtained. Scale bar = 100 μm (A and D).

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