Fig. 3: Knockout of CHD1L significantly blocked the autophagic flux induced by starvation in HCC cells.
From: CHD1L augments autophagy-mediated migration of hepatocellular carcinoma through targeting ZKSCAN3
A WB analysis for starvation-induced changes in LC3 conversion in Dox (−/+)-induced Huh7 and QGY-7703-iKO cells. B WB analysis of changes induced by CHD1L ablation in LC3 conversion in Huh7 and QGY-7703-iKO cells treated with Baf A1 at indicated concentration. C Representative images showing starvation-induced mRFP-LC3 and GFP-mRFP-LC3 puncta accumulation in response to CHD1L deletion in Huh7 and QGY-7703-iKO cells. Quantification of mRFP-LC3 and GFP-mRFP-LC3 puncta per cell. Bar = 10 μM (*p < 0.05; **p < 0.01, ns No significant; Student’s t test; Data indicate mean ± SD; n = 5). D Ultrastructural evidence of autophagy induced by starvation in the indicated cells with CHD1L expression or ablation. The AVd indicate late/degradative autophagolysosomes. Magnification ×10,000–40,000, scale bar represents as shown. E Quantification for autophagic vacuoles as described in “Materials and methods”. (***p < 0.001, Student’s t test; Data indicate mean ± SD; n = 10). Images/immunoblots are representative of three independent experiments.
