Fig. 1: Ela treatment induces fat loss in ob/ob mice.
From: Elaiophylin reduces body weight and lowers glucose levels in obese mice by activating AMPK
A Food intake of ob/ob mice treated with DMSO or 5 mg/kg Ela. n = 6. B Body weight of ob/ob mice intraperitoneally injected with DMSO or Ela every three days, n = 6. C Body composition of ob/ob mice treated as in 1B for two weeks was measured by mice MRI system, n = 6. D Representative hematoxylin and eosin staining from liver, BAT, EWAT, and IWAT in ob/ob mice treated as in 1B for two weeks. BAT, brown-adipose tissue; IWAT, inguinal white-adipose tissue; EWAT, epididymal white adipose tissue. Scale bar, 50 μm. E Plasma triglyceride (TG), HDL, LDL, cholesterol, and AST and ALT were measured in ob/ob mice treated with DMSO or Ela for two weeks, n = 6. F Oxygen-consumption levels and CO2 production over a 24-h period in ob/ob mice treated with DMSO or Ela after two weeks, n = 6. G, H Rectal body temperature of ob/ob mice treated with DMSO or Ela for two weeks at room temperature or during cold exposure. Statistically significant differences in H were compared with pair-fed group, n = 6. I, J Fast and random glucose level of ob/ob mice treated as in 1B for two weeks. Fasting-glucose level recorded after a 6-h fast. Random glucose level was chased for 26 h n = 6. K Glucose tolerance test (GTT) and insulin-tolerance test (ITT) on ob/ob mice treated as in 1B after two weeks n = 6. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 for the indicated comparisons by one-way ANOVA with Tukey tests, except for B, H, J, K, two-way ANOVA and F, G, two-tailed unpaired Student’s t-test. ns = not significant.
