Fig. 5: Ela activates AMPK through LKB1.
From: Elaiophylin reduces body weight and lowers glucose levels in obese mice by activating AMPK
A Mouse embryonic fibroblasts (MEFs) were pretreated with 1 μM bafilomycin A1 at first and then cells were treated with 1 μM Ela for the indicated time. Cells were then lysed and applied for WB. B p18+/+ and p18−/− MEFs were treated with 1 μM Ela for the indicated time, then cells were harvested and followed by WB. C Purified GST-CAMKK, His-AMPK α/β/γ, and GST-ACC (30–100AA) were incubated with Ela at the indicated concentration in the kinase buffer for 30 min. The reaction was terminated by addition of SDS-loading buffer and was analyzed by WB. Compound C was used as a negative control. D Purified GST-CAMKK and His-AMPK α/β/γ were incubated with or without Ela in the kinase buffer for 30 min. The reaction was terminated by addition of SDS loading buffer and was analyzed by WB. E LKB1+/+ and LKB1−/− MEFs were treated with 1 μM Ela for the indicated time, then cells were harvested and applied for WB. F Purified FLAG-LKB1 and His-AMPK α/β/γ were incubated with or without Ela in the kinase buffer for 30 min. The reaction was terminated by addition of SDS-loading buffer and was analyzed by WB.
