Fig. 5: Exosomes derived from GOLPH3-overexpressing cells regulate NSCLC metastasis and a stem cell-like phenotype via activation of the WNT/β-catenin signaling pathway. | Cell Death & Disease

Fig. 5: Exosomes derived from GOLPH3-overexpressing cells regulate NSCLC metastasis and a stem cell-like phenotype via activation of the WNT/β-catenin signaling pathway.

From: GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer

Fig. 5

a GSEA of publicly available NSCLC profiles (REACTOME_SIGNALING_BY_WNT) suggests that GOLPH3 mRNA expression is associated positively with the activation of WNT signaling. b Luciferase-reporter assays of TOP/FOP transcriptional activity in the indicated NSCLC cells. c GO analysis of differentially expressed genes identified by IP-MASS analysis of SFB-vector and SFB-GOLPH3 cells. d Electron micrograph of exosomes isolated from GOLPH3-overexpressing and vector control cells (left; scale bar, 200 nm). Western blot analysis of CD9 and CD81 levels in exosomes of GOLPH3-overexpressing and vector control cells (right). e, f Migration assay of the GOLPH3-silenced cell line treated with exosomes from A549-GOLPH3 cells (e) or NCI-H460-GOLPH3 cells (f) (left). The number of cells that migrated to the lower Transwell chamber, *P < 0.05, right. g, h Migration assay of A549-GOLPH3 cells (g) and NCI-H460-GOLPH3 cells (h) treated with GW4869 (left). Number of cells that migrated to the lower Transwell chamber; *P < 0.05, right. i Real-time PCR analysis of the mRNA expression of metastasis-related markers in GOLPH3-overexpressing and vector cells treated with GW4869.

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