Fig. 6: WNT3A in exosomes derived from GOLPH3-overexpressing cells enhances NSCLC cell metastasis and a stem cell-like phenotype. | Cell Death & Disease

Fig. 6: WNT3A in exosomes derived from GOLPH3-overexpressing cells enhances NSCLC cell metastasis and a stem cell-like phenotype.

From: GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer

Fig. 6

a Western blot analysis of WNT3A levels in exosomes derived from GOLPH3-overexpressing and vector control cells (left); quantification of WNT3A expression (right). b ELISA of WNT3A levels in GOLPH3-overexpressing, GOLPH3-silenced, or vector control cells. c ELISA of WNT3A levels in exosomes derived from GOLPH3-overexpressing and vector control cells. d Western blot analysis of WNT3A levels in exosomes derived from GOLPH3-overexpressing cells treated with GW4869 (left); quantification of WNT3A expression (right). e ELISA of WNT3A levels in GOLPH3-overexpressing cells treated with GW4869. f ELISA of WNT3A levels in exosomes of GOLPH3-overexpressing cells treated with GW4869 inhibitors. g, h Real-time PCR analysis of the mRNA expression stemness-related markers (g) and metastasis-related markers (h) in GOLPH3-silenced and vector control cells treated with exosomes or recombinant WNT3A protein.

Back to article page