Fig. 8: GOLPH3 enhances the secretion of CKAP4-containing exosomes to induce NSCLC metastasis and a cancer stem cell-like phenotype. | Cell Death & Disease

Fig. 8: GOLPH3 enhances the secretion of CKAP4-containing exosomes to induce NSCLC metastasis and a cancer stem cell-like phenotype.

From: GOLPH3/CKAP4 promotes metastasis and tumorigenicity by enhancing the secretion of exosomal WNT3A in non-small-cell lung cancer

Fig. 8

a Query results for gene symbol CKAP4 in ExoCarta. b Western blot analysis of GOLPH3, WNT3A, and CKAP4 levels in exosomes and CKAP4 levels in the plasma membrane (PM) or total lysate of GOLPH3-overexpressing or GOLPH3-silenced cells. c Western blot analysis of CKAP4 levels in the PM of GOLPH3-overexpressing or control cells treated with or without GW4869. d Co-IP analysis of the interactions between CKAP4 and WNT3A in exosomes derived from GOLPH3-overexpressing cells. e ELISA of WNT3A levels in GOLPH3-overexpressing and vector control cells treated with siCKAP4 or siRNA-vector. f Model of the mechanism by which GOLPH3 overexpression promotes the secretion of CKAP4-containing exosomes and the transfer of WNT3A, to induce activation of the WNT signaling pathway and thereby, enhance metastasis and a stem cell-like phenotype in NSCLC.

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