Fig. 7: NPRA promoted tumor angiogenesis by binding and protecting HIF-1α against protein degradation.
From: Natriuretic peptide receptor a promotes gastric malignancy through angiogenesis process
A The proteins that bound to NPRA were determined by immunoprecipitation/mass spectrum (IP/MS) analysis, which indicated that NPRA could bind to HIF-1α at high binding scores. B Co-IP experiments demonstrated that NPRA immunoprecipitated with anti- HIF-1α antibody, and HIF-1α immunoprecipitated with anti- NPRA antibody. C, D Western blot analysis of HIF-1α protein stability using cycloheximide (CHX, an inhibitor of protein synthesis) when knockdown of NPRA. E AGS cells transfected with shNPRA and shCTL were treated with MG132, HIF-1α, and NPRA protein levels were detected by western blot.
