Fig. 2: Decrease of circUBE2J2 expression promoted the proliferation of HCC cells in vitro and in vivo. | Cell Death & Disease

Fig. 2: Decrease of circUBE2J2 expression promoted the proliferation of HCC cells in vitro and in vivo.

From: Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression

Fig. 2

A The expression level of circUBE2J2 in the subcellular fractions of HepG2 cells was detected by qRT-PCR. NEAT1 and GAPDH were used as nuclear and cytoplasmic markers, respectively. B The overexpression and knockdown efficiency of circUBE2J2 were examined by qRT-PCR. C–E CCK-8 (C), colony formation assay (D), and EDU (E) were used to evaluate HCC cells proliferation after circUBE2J2 overexpression or knockdown. Scale bar: white bar, 200 μm. F Flow cytometry detection showing the percentages of cells in the G1, S, or G2 phase in both HepG2 and MHCC97H cells. G–L HCC cells were subcutaneously injected into nude mice, and tumor growth curves and tumor volume were plotted. Data are presented as mean ± SD; **p < 0.05; **p < 0.01; vs. NC. M Expression levels of Ki67 were observed in subcutaneous tumor tissues by IHC. The data are represented as the mean ± SD, n = 3. *p < 0.05; **p < 0.01; NS no significance. OE overexpression; sh-circ transfected with lentivirus for circUBE2J2-specific shRNA; sh-NC transfected with control lentivirus.

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