Fig. 8: CircUBE2J2 inhibited HCC progression through the circUBE2J2/miR-370-5p/KLF7 axis. | Cell Death & Disease

Fig. 8: CircUBE2J2 inhibited HCC progression through the circUBE2J2/miR-370-5p/KLF7 axis.

From: Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression

Fig. 8

A KLF7 expression in the mRNA level was measured by qPCR in HCC cells after circUBE2J2 knockdown. B After circUBE2J2 knocking down, miR-370-5P inhibitor or inhibitor nc were added to HLF and HepG2 cell culture. The KLF7 protein expression level was analyzed by western blot. C The cell proliferation in HCC cells with the reduced expression of miR-370-5P was assessed by a CCK-8 assay. D The proliferation ratio of HCC cells was measured by EdU incorporation. E The migration abilities in HCC cells with the reduced expression of miR-370-5P were evaluated via a transwell assay. F After miR-370-5P overexpression, KLF7 overexpression vector was added to MHCC97H and HLF cell culture. KLF7 expression in the mRNA level was measured by qPCR. G The KLF7 protein expression level was analyzed by western blot. H The cell proliferation in HCC cells was assessed by a CCK-8 assay. I Colony formation assays show the proliferation. J Transwell migration assays. Data are presented as mean ± SD, n = 3. *p < 0.05, **p < 0.01; NS no significance. sh-circ transfected with lentivirus for circUBE2J2-specific shRNA; sh-NC transfected with control lentivirus.

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