Fig. 3: HCG15 carried by exosomes was transferred to cardiomyocytes after hypoxia. | Cell Death & Disease

Fig. 3: HCG15 carried by exosomes was transferred to cardiomyocytes after hypoxia.

From: Loss of exosomal LncRNA HCG15 prevents acute myocardial ischemic injury through the NF-κB/p65 and p38 pathways

Fig. 3

A qRT-PCR detected the expression of HCG15 in hypoxic AC16 cells. B Exosomes isolated from hypoxic AC16 cells are shown by TEM. C Western blotting was used to detect the expression of biomarkers in the exosome (Exo) and supernatant fractions (Sup) of the medium from AC16 cells after 8 h of hypoxia. D qRT-PCR was used to detect the level of HCG15 in exosomes and their producers (AC16 cells) after hypoxic treatment. E Confocal microscopy showed that PKH67-labeled exosomes from hypoxic AC16 cells were taken up by AC16 cells at different times. The nuclei were stained with DAPI; green: PKH67 (the magnification was × 400). F Expression of HCG15 in AC16 cells after incubation with Exo-N and Exo-H at different times was detected by qRT-PCR. *p value < 0.05 vs 0 h, #p value < 0.05 vs Exo-N. Each assay was performed in triplicate.

Back to article page