Fig. 4: LncRNA HCG15 in exosomes released from hypoxic cells contributed to cardiomyocyte apoptosis and the production of inflammatory cytokines. | Cell Death & Disease

Fig. 4: LncRNA HCG15 in exosomes released from hypoxic cells contributed to cardiomyocyte apoptosis and the production of inflammatory cytokines.

From: Loss of exosomal LncRNA HCG15 prevents acute myocardial ischemic injury through the NF-κB/p65 and p38 pathways

Fig. 4

A qRT-PCR was performed to determine the expression of HCG15 in AC16 cells after transfection with HCG15 siRNAs. B qRT-PCR was performed to detect the expression of HCG15 in AC16 cells after transfection with HCG15 siRNA and subsequent incubation with Exo-H. C MTT assays detected the proliferation of AC16 cells after transfection with HCG15 siRNA and subsequent incubation with Exo-H. TUNEL staining (D) and flow cytometry (E) were performed to measure the apoptosis of AC16 cells after the indicated treatment. F ELISAs were used to measure the production of inflammatory cytokines in AC16 cells after the indicated treatment. *p value < 0.05, ***p value < 0.001. Each assay was performed in triplicate.

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