Fig. 3: Re-expression of CGI-58 by rAAV9 injection reverses cardiac remodeling and dysfunction in CGI-58 knockout mice.
From: Cardiac-specific CGI-58 deficiency activates the ER stress pathway to promote heart failure in mice
A M-model echocardiography of the left ventricle (upper). Measurement of EF% and FS% (lower, n = 8). B Heart size (upper), heart weight, and HW/TL ratio (lower, n = 8). Scale bar 2 mm. C FITC-labeled WGA staining of the heart sections (upper). Quantification of the relative myocyte cross-sectional area (lower, n = 5). D qPCR analyses of ANP, BNP, and Myh7 mRNA levels (n = 5). E Masson’s Trichrome staining of heart sections (left) and the quantification of fibrotic area (right, n = 5). F qPCR analyses of collagen I and collagen III mRNA levels (n = 5). G Oil Red O staining of heart sections (left) and the quantification of lipid accumulation (n = 5). H Measurement of TG content in the heart (n = 5). I DHE staining of heart sections (left) and the quantification of ROS level (right, n = 5). Scale bar 50 μm. Data are presented as mean ± SEM, and n represents the number of samples or animals. *P < 0.05, **P < 0.01, ***P < 0.001.
