Fig. 1: Quantitative analysis of necroptosis phosphoproteome in TNFα-stimulated FADD-deficient Jurkat cells. | Cell Death & Disease

Fig. 1: Quantitative analysis of necroptosis phosphoproteome in TNFα-stimulated FADD-deficient Jurkat cells.

From: Quantitative analysis of phosphoproteome in necroptosis reveals a role of TRIM28 phosphorylation in promoting necroptosis-induced cytokine production

Fig. 1

A PCA plot based on log2Ratios of FADD-deficient Jurkat cells treated with TNFα vs. that of vehicle only or (TNFα + Nec-1s) vs. that of vehicle only at the indicated time points of treatments. B Scatter plots comparing log2Ratios of FADD-deficient Jurkat cells treated with TNFα vs. that with vehicle only and FADD-deficient Jurkat cells treated with (TNFα + Nec-1s) vs. with vehicle only. The green and blue dots represent phosphosites with log2Ratios of FADD-deficient Jurkat cells treated with TNFα vs. that with vehicle only, either ≥0.485 or ≤ -0.485 and p-value < 0.05, respectively, but were not affected by the addition of Nec-1s. The phosphosites that were affected by treatment with TNFα and inhibited or rescued over 50% by Nec-1s were colored by red or yellow, respectively. C The consensus motif analysis of phosphorylation sites that are dependent on RIPK1 kinase activation. The pre-aligned sequences of 15 residues or less containing phosphosites upregulated by TNFα and inhibited by treatment with Nec-1s were subjected to motif analysis using the tool in PhosphositePlus®. D Pathway analysis of phosphoproteins. Phosphosites that were up or downregulated in TNFα-treated cells, which were inhibited or rescued by Nec-1s were subjected to GO analysis. The heat map displays a cluster of significantly enriched pathways. The color scale is defined based on –log10 (p-value). The pathways highlighted in red and blue are corresponding to those related to inflammation and gene expression, respectively. E Network analysis of 247 phosphoproteins containing 299 phosphosites, which were up- or downregulated by TNFα and inhibited or rescued by Nec-1s at the time point of 4 h. MCODE algorithm was applied to identify the neighborhoods where proteins were densely connected. Each MCODE network is assigned a unique color. GO enrichment analysis was applied to each MCODE network to assign biological functions to the network component. Circles, triangles and diamonds represent proteins containing Nec-1s inhibited, rescued and both inhibited and rescued phosphosites, respectively. TNFα, 20 ng/mL; Nec-1s, 10 μM.

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