Fig. 3: IKBKBAS is a positive regulator of NF-κB signaling pathway. | Cell Death & Disease

Fig. 3: IKBKBAS is a positive regulator of NF-κB signaling pathway.

From: Novel cytoplasmic lncRNA IKBKBAS promotes lung adenocarcinoma metastasis by upregulating IKKβ and consequential activation of NF-κB signaling pathway

Fig. 3

A The relative expression of IKKβ in A549 cells with ectopic expression of IKBKBAS were assayed by qRT-PCR. B Western blot analysis for the expression of IKKβ, p-IKKβ, IκBα, and p-IκBα in A549 cells with ectopic expression of IKBKBAS. C The relative expression of IKKβ in HCC827 cells with knockdown of IKBKBAS assayed by qRT-PCR. D Western blot analysis for the expression of IKKβ, p-IKKβ, IκBα, and p-IκBα in HCC827 cells with knockdown of IKBKBAS. E Activity of NF-κB in A549 cells with ectopic expression of IKBKBAS and activity of NF-κB in HCC827 cells with knockdown of IKBKBAS were detected by EMSA. F The relative expression of target genes of NF-κB were assayed by qRT-PCR in A549 cells, including IL-1α/6/8, MMP9/2, c-myc, CCL20. The A549 cells were treated with ectopic expression of IKBKBAS, and with treatment of NF-κB inhibitor PDTC and IKKβ inhibitor TPCA-1, respectively. G MTT assay showing the effect of IKBKBAS on cell proliferation in A549 cells with PDTC or TPCA-1 treatment. H Plate colony formation assays showing the effect of IKBKBAS on colonizing ability in A549 cells with PDTC or TPCA-1 treatment. I Transwell assays exhibited the effect of IKBKBAS on invasion and migration ability in A549 cells with PDTC or TPCA-1 treatment.Data show mean ± SD, n ≥ 3, *P < 0.05, **P < 0.01, ***P < 0.001. si-1: si-288; si-2: si-449; si3: si-288+si-449.

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