Fig. 4: Cytoplasmic IKBKBAS upregulates IKKβ at post-transcriptional level. | Cell Death & Disease

Fig. 4: Cytoplasmic IKBKBAS upregulates IKKβ at post-transcriptional level.

From: Novel cytoplasmic lncRNA IKBKBAS promotes lung adenocarcinoma metastasis by upregulating IKKβ and consequential activation of NF-κB signaling pathway

Fig. 4

A Confocal FISH images showing cytoplasmic localization of IKBKBAS in A549, H1299, and HCC827 cells, respectively. The nuclei were stained with DAPI, U6 snRNA, and 18S rRNA was used as a nuclei and cytoplasmic marker, respectively. IKBKBAS-AS were used as negative control. Scale bar: 10 μm. B Cell nuclear/cytoplasmic fractionation and qRT-PCR assay showing the cellular distribution of IKBKBAS in A549, H1299, and HCC827 cells, respectively. U6 and GAPDH were used as separation quality standards and endogenous controls. C The kinetics of IKKβ mRNA in A549, H1299, and HCC827 cells treated with Act-D were assayed by qRT-PCR, respectively. D The kinetics of IKKβ mRNA in A549 cells with ectopic IKBKBAS expression and Act-D treatment were assayed by qRT-PCR. E Luciferase reporter assay showing the effect of IKBKBAS on promoter activity of IKBKB gene in A549 cells with ectopic expression of IKBKBAS. F Luciferase reporter assay showing the effect of IKBKBAS on promoter activity of IKBKB gene in HCC827 cells with knockdown of IKBKBAS expression.

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