Fig. 7: Reciprocal positive regulation between IKBKBAS and NF-κB. | Cell Death & Disease

Fig. 7: Reciprocal positive regulation between IKBKBAS and NF-κB.

From: Novel cytoplasmic lncRNA IKBKBAS promotes lung adenocarcinoma metastasis by upregulating IKKβ and consequential activation of NF-κB signaling pathway

Fig. 7

A Schematic representation of the predicted binding sites for NF-κB on IKBKBAS promoter for ChIP assay. B Luciferase reporter assay showing the relative promoter activity of IKBKBAS in A549 cells treated with TNFα 40 ng/ml for 12, 24 h, respectively. C The expression of IKBKBAS in A549 cells treated with TNFα 40 ng/ml for 12, 24, 48 h was detected by qRT-PCR, respectively. D Expression of IKBKBAS detected in A549 cells and HCC827 cells with treatment of PDTC (10, 20, 40 μM) for 60 min, 120 min by qRT-PCR. E The interaction between p65 and two κB sites on IKBKBAS promoter were assayed by ChIP experiments. The anti-p65 or IgG antibody (negative control) or anti-RNA pol II (positive control) in A549 cells and HCC827 cells treated with TNFα (40 ng/ml) for 12 h were performed. Left: the RT-PCR results (lane M: marker; lanes 1–3 & 5–7: IKBKBAS promoter DNA fragment containing 2 κB sites, 161 bp; lane 4: GAPDH promoter DNA fragment: 166 bp); right: qRT-PCR results. Y axis represents the % input of the promoter fragments captured by the two different antibodies. Data show mean ± S.E.M., n ≥ 3, *P < 0.05, **P < 0.01, ***P < 0.001.

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