Fig. 3: AE-specific CD8+ T cells can be found in naïve mice. | Cell Death & Disease

Fig. 3: AE-specific CD8+ T cells can be found in naïve mice.

From: CD8+ T cells specific for cryptic apoptosis-associated epitopes exacerbate experimental autoimmune encephalomyelitis

Fig. 3

A, B Splenocytes from C57BL/6 naïve mice (n = 5) were analyzed by flow cytometry. A Representative stainings (left) and cumulative analysis (right) of CD8+ T cells specific for the indicated single peptides. FMO, fluorescence-minus-one control. *P < 0.05, **P < 0.01, by Mann–Whitney test, compared to the FMO. B Representative stainings (left) and cumulative analysis (right) of CD44+ cells in the shown subsets. *P < 0.05, **P < 0.01, by Mann–Whitney test, compared to the total CD8+ T cells. Overall this analysis was repeated thrice. C CFSE profiles (left) and percentages of proliferating cells (right) in gated naïve or memory CD8+ T cells that have been stimulated for 4 days with irradiated splenocytes, anti-CD28, plus the indicated stimuli. The data are from a single experiment representative of three independent experiments. Bars represent means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.005, by Mann-Whitney test, compared to “no peptide” in each subgroup.

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