Fig. 5: Immunization with OVA257-264, but not with MYH9761-768, induces antigen-specific CD8+ T cell responses. | Cell Death & Disease

Fig. 5: Immunization with OVA257-264, but not with MYH9761-768, induces antigen-specific CD8+ T cell responses.

From: CD8+ T cells specific for cryptic apoptosis-associated epitopes exacerbate experimental autoimmune encephalomyelitis

Fig. 5

C57BL/6 mice were immunized with OVA257-264 (n = 8), with MYH9761-768 (n = 8) or with no peptide (n = 5), and flow cytometry analysis was performed. Representative stainings (A) and percentages (B) of OVA257-264-specific (blue) or MYH9761-768-specific (red) cells in mice immunized with the respective peptides or with saline (no peptide), in alum and MPL as adjuvants. Samples with insufficient numbers of dextramer+ or tetramer+ events were not included in the analysis. C Splenocytes were challenged ex vivo for 48 h with the indicated stimuli and IFN-γ was measured in the supernatants through ELISA. The data are from a single experiment representative of two independent experiments. Bars represent means ± SEM. **P < 0.01, ***P < 0.005, by Mann–Whitney test.

Back to article page