Fig. 1: p32 silencing suppresses mouse B16F10 and human A375 melanoma cell tumorigenesis in vitro. | Cell Death & Disease

Fig. 1: p32 silencing suppresses mouse B16F10 and human A375 melanoma cell tumorigenesis in vitro.

From: p32 promotes melanoma progression and metastasis by targeting EMT markers, Akt/PKB pathway, and tumor microenvironment

Fig. 1

A Immunoblot and relative mRNA expression showing the level of p32 in ctrl shRNA and p32 shRNA-treated B16F10 cells. B Immunoblot and relative mRNA expression showing the level of p32 in ctrl shRNA and p32 shRNA-treated A375 cells. C Invasion assay showing the number of invading cells through transwell chamber 12 h after stimulation with 5% FBS; D cell proliferation quantified by MTT assay after 24 and 48 h; E colony formation assay showing colony numbers per well along with graphical representation. F Bar graph quantifying the percentage of annexin V and PI staining in B16F10 and A375 cells and G wound-healing showing 0, 12, and 24 h post wounding and its graphical representation showing percentage wound closure in ctrl shRNA and p32 shRNA-treated B16F10 and A375 cells. All data are represented as mean ± SD. Student’s t-test was used for all statistical analyses, n = 3 (****P < 0.0001,***P < 0.001, **P < 0.01, *P < 0.05).

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