Fig. 3: p32 silencing induces melanoma cell tumorigenesis via Akt/PKB pathway in melanoma cells. | Cell Death & Disease

Fig. 3: p32 silencing induces melanoma cell tumorigenesis via Akt/PKB pathway in melanoma cells.

From: p32 promotes melanoma progression and metastasis by targeting EMT markers, Akt/PKB pathway, and tumor microenvironment

Fig. 3

A Immunoblot showing the phosphorylation levels of p-Akt, p-p38, and p-ERK in B16F10 and A375 melanoma cells after p32 silencing. B Wound-healing/scratch assay showing cell migration; C cell proliferation at 24 and 48 h and D invasion assay showing the number of invading cells through transwell chamber 12 h after stimulation with 5% FBS in ctrl shRNA and p32 shRNA-treated B16F10 and A375 cells with and without Akt/PKB inhibitor LY294002 treatment. All the data are represented as mean ± SD. Student’s t-test was used for all statistical analyses, n = 3 (***P < 0.001, **P < 0.01, *P < 0.05).

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