Fig. 3: Nrmt1−/− mice exhibit short- and long-term memory impairment and hyperactivity. | Cell Death & Disease

Fig. 3: Nrmt1−/− mice exhibit short- and long-term memory impairment and hyperactivity.

From: Age-related neurodegeneration and cognitive impairments of NRMT1 knockout mice are preceded by misregulation of RB and abnormal neural stem cell development

Fig. 3

Two-trial Barnes maze spatial memory tasks show that Nrmt1−/− mice spend significantly less time exploring the correct hole than WT mice at a 6 weeks and b 3 months. c At 6 months, Nrmt1−/− mice spend significantly more time at the incorrect holes. d The spatial memory indexes (SMI) calculated from the two-trial tasks indicate short-term memory loss at all three ages. SMI = (time exploring correct hole − time exploring incorrect holes)/(total exploration time). e The SMIs calculated from the ten-trial tasks also indicate long-term memory loss at all three ages. Although Nrmt1−/− mice are able to learn the correct hole when given ten training trials at f 6 weeks, g 3 months, and h 6 months, the ten-trial Barnes maze also shows Nrmt1−/− mice spend significantly less time exploring the correct hole at i 6 weeks and j 3 months, and significantly more time at incorrect holes at k 6 months. l Rotarod assays show a small motor impairment in Nrmt1−/− mice at 6 months. Open-field assays show Nrmt1−/− mice travel at m higher average speeds for n–p longer distances than WT mice. *p < 0.05, **p < 0.005, ***p < 0.0005, and ****p < 0.0001 as determined by a–c, i–k two-way ANOVA or d–h, l–p unpaired t-test, n = 6–10. Error bars represent mean ± SEM.

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