Fig. 3: Effect of GPER on the apoptosis of crypt cell in 5-FU induced CIM. | Cell Death & Disease

Fig. 3: Effect of GPER on the apoptosis of crypt cell in 5-FU induced CIM.

From: Activation of G protein coupled estrogen receptor prevents chemotherapy-induced intestinal mucositis by inhibiting the DNA damage in crypt cell in an extracellular signal-regulated kinase 1- and 2- dependent manner

Fig. 3

CIM model was induced by i.p. injection 5-FU (30 mg/kg/day) for 5 days. G-1 (0.03 mg/kg/day) and/or G15 (0.3 mg/kg/day) were administrated intraperitoneally with 5-FU together. G-1 was administrated alone or with G15 together in CIM model to test the effect of GPER activation. G15 was used alone to test the effect of endogenous GPER blocking. On the 5th day, the ileum was collected for immunofluorescence, immunohistochemical staining and western blot. Data were expressed as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001). a Representative western blots photographs for Cleaved caspase-3 and Caspase-3 expression following G-1 administration with or without G15 in the CIM model. b Statistical analysis of Cleaved caspase-3 expression in ileum tissue within four subgroups to show the effect of GPER activation on apoptosis in the CIM model (n = 4). c Representative western blots photographs for Bcl-2 and Bax expression following G-1 administration with or without G15 in the CIM model. d Statistical analysis of Bcl-2, Bax expression and Bax/ Bcl-2 ratio within four subgroups to show the effect of GPER activation on apoptosis in the CIM model (n = 4). e Representative western blots photographs for Cleaved caspase-3, Caspase-3, Bcl-2, Bax expression following G15 administration alone in the CIM model. f Statistical analysis of Cleaved caspase-3 expression in ileum tissue within three subgroups to show the effect of endogenous GPER blocking with G15 on apoptosis in the CIM model (n = 4). g Statistical analysis of Bcl-2, Bax expression and Bax/ Bcl-2 ratio in ileum tissue within three subgroups to show the effect of endogenous GPER blocking with G15 on apoptosis in the CIM model (n = 4). h Representative images for TUNEL staining in ileum following G-1 administration with or without G15 in the CIM model (scale bars: 50 μm). i Statistical graph for the number of TUNEL + cells per crypt within four subgroups to show the effect of GPER activation on apoptosis of crypt cell in the CIM model. At least 20 crypts were counted randomly for each sample, and the mean value was calculated for single sample (n = 4).

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