Fig. 7: Role of ERK1 / 2 activity in GPER protection against 5-FU induced DNA damage in IEC-6 cells. | Cell Death & Disease

Fig. 7: Role of ERK1 / 2 activity in GPER protection against 5-FU induced DNA damage in IEC-6 cells.

From: Activation of G protein coupled estrogen receptor prevents chemotherapy-induced intestinal mucositis by inhibiting the DNA damage in crypt cell in an extracellular signal-regulated kinase 1- and 2- dependent manner

Fig. 7

IEC-6 cells were stimulated under 10−4 mol/L 5-FU for 48 h or 96 h with or without G-1 application (10−7 mol/L). The selective ERK1/2 inhibitor PD0325901(5 × 10−3 mol/L) was administrated to block the ERK1/2 phosphorylation. Data were expressed as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001). a Representative western blots photographs for P-ERK1/2 and cyclin D1 in cultured IEC-6 cells exposure to 5-FU for 48 h in the presence or absence of G-1. b Representative western blots photographs for P-ERK1/2 and cyclin D1 in cultured IEC-6 cells exposure to 5-FU for 96 h in the presence or absence of G-1. c Statistical analysis of P-ERK1/2 expressions in IEC-6 cells exposed to 5-FU for 48 h or 96 h in the presence or absence of G-1 (n = 3). d Statistical analysis of cyclin D1 expressions in IEC-6 cells exposed to 5-FU for 48 h or 96 h in the presence or absence of G-1 (n = 3). e Representative images of comet assay for IEC-6 cells showing the effect of PD0325901 on G-1 inhibition of 5-FU induced DNA damage (scale bars: 100 μm). f Statistical graph of comet assay. Tail length, tail DNA percentage, tail moment and olive tail moment of IEC-6 within four subgroups to show the effect of PD0325901 on G-1 inhibition of 5-FU induced DNA damage. The experiment was repeated three times independently, 50 cells of each time were analyzed (n = 150). g Effect of PD0325901 on G-1 protection of P-ERK1/2 activities in 5-FU treated cells (n = 3).

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