Fig. 3: Ionizing radiation induces ER-phagy in ER+ breast cancer cells. | Cell Death & Disease

Fig. 3: Ionizing radiation induces ER-phagy in ER+ breast cancer cells.

From: Ionizing radiation downregulates estradiol synthesis via endoplasmic reticulum stress and inhibits the proliferation of estrogen receptor-positive breast cancer cells

Fig. 3

MCF7 and T47D cells were irradiated with X-rays at different doses. Twenty-four hours after irradiation, the expression of endoplasmic reticulum stress marker proteins increased (A). Ionizing radiation upregulated the expression of XBP1s and IRE1α (B, C). The expression of XBP1s increased in MCF7 tumors after irradiation (D). The expression of the key autophagic proteins LC3B and Beclin1 increased, and P62 expression was attenuated (E). The expression of LAMP1 and LAMP2 was augmented (F). Irradiation induced morphologic shrinkage of the ENR and lysosomal aggregation increased (G). The colocalization of CNX/LC3B (H) and CNX/LAMP2 (I) increased with irradiation (mean ± SD of triplicate assessments, Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001).

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