Fig. 4: Ionizing radiation downregulates the expression of CYP19A. | Cell Death & Disease

Fig. 4: Ionizing radiation downregulates the expression of CYP19A.

From: Ionizing radiation downregulates estradiol synthesis via endoplasmic reticulum stress and inhibits the proliferation of estrogen receptor-positive breast cancer cells

Fig. 4

MCF7 and T47D cells were irradiated with X-rays at different doses. Twenty-four hours after irradiation, the secretion of estradiol decreased (A). Irradiation downregulated CYP19A expression in vivo and in vitro (B–D). CYP19A−/− MCF7 cell clonal formation was significantly slowed (E). Irradiation reduced the colocalization of CNX and CYP19A (F), and increased the colocalization of CYP19A and lysosomes (G). Irradiation inhibited CYP19A transport from the EnR to the Golgi complex (H). 4-PBA rescued ionizing radiation-induced cell death (I). In the case of endoplasmic reticulum stress (ERS) inhibition, the expression of CYP19A and ERα was not significantly decreased after irradiation and autophagy was also inhibited (J). The expression of CYP19A declined by ionizing radiation and ERS inducer co-treatment (K). Ionizing radiation induced ERS and autophagy in ER− breast cancer cell (L) (mean ± SD of triplicate assessments, Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001).

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