Fig. 6: Ectopic ARSD overexpression activates Hippo/YAP pathway in breast cancer cells. | Cell Death & Disease

Fig. 6: Ectopic ARSD overexpression activates Hippo/YAP pathway in breast cancer cells.

From: ARSD, a novel ERα downstream target gene, inhibits proliferation and migration of breast cancer cells via activating Hippo/YAP pathway

Fig. 6

A, B Western blotting verifies that ARSD overexpression activates Hippo/YAP pathway in MDA-MB-231 and BT-549 cells. C ARSD knocking down inactivates Hippo/YAP pathway in MCF-7 cells. All molecular weights are in KDa. All experiments were repeated three times independently, yielding similar results, representative images are shown. D Cell proliferation analysis of negative control (MDA-MB-231/ARSD/shNC cells) and MDA-MB-231/ARSD/shKibra cells is detected every 24 h after plating (day 0) by using CCK-8 assay. E Transwell assay of MDA-MB-231/ARSD/shNC cells and MDA-MB-231/ARSD/shKibra cells. F Wound healing assay of MDA-MB-231/ARSD/shNC cells and MDA-MB-231/ARSD/shKibra cells. G Colony formation of MDA-MB-231/ARSD/shNC cells and MDA-MB-231/ARSD/shKibra cells on a plastic substrate. The colony formation rate was quantified using ImageJ software 14 days after plating; error bars are ±SEM (*p < 0.05, **p < 0.01, ***p < 0.001).

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