Fig. 2: Mitochondrial Drp1 recognizes mPTP channels through binding with BAX and PiC after hypoxia. | Cell Death & Disease

Fig. 2: Mitochondrial Drp1 recognizes mPTP channels through binding with BAX and PiC after hypoxia.

From: Mitochondrial Drp1 recognizes and induces excessive mPTP opening after hypoxia through BAX-PiC and LRRK2-HK2

Fig. 2

A The schematic diagram showing the proteins related to mPTP channel under current cognition. B Co-IP results for the combination of Drp1 and BAX in total, cytoplasmic and mitochondrial fractions of VSMCs after hypoxia. C Co-IP results for the combination of Drp1 and PiC in total, cytoplasmic and mitochondrial fractions of VSMCs after hypoxia. D Co-IP results for the combination of Drp1 and other mPTP related proteins, including HK2, CK, ANT, ATPase, VDAC and CypD, after hypoxia. E Representative confocal images of mPTP opening in VSMCs after hypoxia and Mdivi-1 (50 μM) treatment under intervening BAX or PiC (bar, 100μm). BAX inhibitor: BAX activation inhibitor 1 (BAI1, 5 μM), PiC inhibitor: N-Ethylmaleimide (NEM, 5 μM), BAX activator: BAX trigger site activator 1 (BTSA1, 15 μM), PiC activator: spingosine-1 phosphate receptor agonist (SEW2871, 15 nM). Relative mPTP opening ratio (fluoresce intensity of MitoTracker/Calcein) in each group was calculated by Image J (n = 5 samples/group). a: P < 0.05 compared with Normal group, b: P < 0.05 compared with Hypoxia group.

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