Fig. 5: Ectopic expression of mouse PD-L1 (mPD-L1) could reverse USP5 knockdown induced tumor growth inhibition.
From: USP5 facilitates non-small cell lung cancer progression through stabilization of PD-L1
A T-cell killing assay of LLC cells transfected with indicated USP5 siRNA or mouse Flag-PD-L1 (Flag-mPD-L1) was performed to as described in “Methods” section, and the cytotoxicity was evaluated by LDH release assay using the culture supernatant. B Western blot analysis of USP5 and PD-L1 expression in LLC cells transfected with indicated constructs. LLC cells expressing control, USP5 shRNA or USP5 shRNA plus Flag-mPD-L1 were implanted into right flank of C57BL6 mice. C Tumor image and tumor growth curve (D) was shown. *P < 0.05 and ***P < 0.001 by two-tailed t-test.
