Fig. 6: Cpeb3 mutation causes POI in mice by downregulation of the oocyte-derived factor, Gdf9. | Cell Death & Disease

Fig. 6: Cpeb3 mutation causes POI in mice by downregulation of the oocyte-derived factor, Gdf9.

From: CPEB3 deficiency in mice affect ovarian follicle development and causes premature ovarian insufficiency

Fig. 6

A Western blot analysis of GAPDH, GDF9, PCNA, and Cleaved-Caspase3 protein expression in 3-month-old WT and Cpeb3-mutant ovaries. GAPDH was used as the internal control. B Western blot analysis of GDF9 and GAPDH in WT and Cpeb3-mutant oocytes. GAPDH was used as the internal control. C Ovarian sections stained for GDF9 at 3-month-old WT and Cpeb3-mutant ovaries. D Gdf9 mRNA levels were performed by qRT-PCR in WT and Cpeb3-mutant oocytes. Gapdh was used as the internal control. Scale bar = 50 μm and 25 μm in C. ***P < 0.001.

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