Fig. 2: Sk-Mel-28 and Ma-Mel-19 human melanoma cell lines express a functional P2X7 receptor. | Cell Death & Disease

Fig. 2: Sk-Mel-28 and Ma-Mel-19 human melanoma cell lines express a functional P2X7 receptor.

From: P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells

Fig. 2

A mRNA expression of P2X7A and P2X7B in Sk-Mel-28 cells evaluated by real time-PCR (N = 4). B mRNA expression of P2X7A and P2X7B in Ma-Mel-19 cells evaluated by real-time PCR (N = 4). P2X7 receptor activity as a channel was evaluated in C Sk-Mel-28 cells and D Ma-Mel-19 cells by measuring variations in [Ca2+]I following stimulation with P2X7R agonist BzATP (300 μM). The P2X7 receptor antagonists A740003 (20 μM) and AZ10606120 (2 μM) completely obliterated the effect of BzATP. P2X7 activity as a large solute (up to 900 Da) pore was evaluated in E Sk-Mel-28 cells and F Ma-Mel-19 cells by measuring ethidium uptake following stimulation with BzATP (300 μM). The P2X7 receptor antagonists A740003 (20 μM) and AZ10606120 (2 μM) blocked the effect of BzATP.

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