Fig. 3: P2X7 antagonists reduce melanoma cell proliferation, anchorage-independent growth, and migration.

A Sk-Mel-28 cells were treated with P2X7 antagonists A740003 (20 μM) and AZ10606120 (2 μM) and counted at time 0 and after 24 and 48 h of incubation. Data are presented as a fold increase of time 0 (N = 10, five independent experiments). B number of colonies formed by Sk-Mel-28 cells in soft agar plates after 20 days of incubation with a vehicle, A740003 (20 μM) or AZ10606120 (2 μM) (N = 6, three independent experiments). C representative pictures showing colonies formed by Sk-Mel-28 cells. D quantification of the migratory ability of Sk-Mel-28 cells expressed as the percentage of the migration area evaluated by scratch closure assay at 24 and 48 h of incubation with vehicle, A740003 (20 μM) or AZ10606120 (2 μM) (N = 6, three independent experiments). E representative images of Sk-Mel-28 cells scratches taken at times 0, 24 and 48. F Ma-Mel-19 cells were treated with P2X7 antagonists A740003 (20 μM) and AZ10606120 (2 μM) and counted at time 0 and after 24 and 48 h of incubation. Data are presented as a fold increase of time 0 (N = 7, in three independent experiments). G number of colonies formed by Ma-Mel-19 cells in soft agar plates after 20 days of incubation with vehicle, A740003 (20 μM) and AZ10606120 (2 μM) (N = 6, three independent experiments). H representative pictures showing colonies formed by Ma-Mel-19 cell. I quantification of the migratory ability of Ma-Mel-19 cells expressed as the percentage of the migration area evaluated by scratch closure assay at 24 and 48 h of incubation with vehicle, A740003 (20 μM) and AZ10606120 (2 μM) (N = 6, three independent experiments). J representative images of Ma-Mel-19 cells scratches taken at times 0, 24, and 48. Data are shown as the mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test.