Fig. 8: Effect of miR-495-3p, miR-376c-3p, and miR-6730-3p transfection on proliferation and migration of Sk-Mel-28 and Ma-Mel-19 cells. | Cell Death & Disease

Fig. 8: Effect of miR-495-3p, miR-376c-3p, and miR-6730-3p transfection on proliferation and migration of Sk-Mel-28 and Ma-Mel-19 cells.

From: P2X7 promotes metastatic spreading and triggers release of miRNA-containing exosomes and microvesicles from melanoma cells

Fig. 8

A Sk-Mel-28 or B Ma-Mel-19 cells were transfected with miRNA mimics for miR-495-3p, miR-376c-3p, miR-6730-3p and scrambled control. Cells were counted for 3 days, starting 24 h after transfection. Data are presented as a fold increase on time 0. C–F Sk-Mel-28 and Ma-Mel-19 cells were transfected with the above-described miRNA mimics or control. The scratch recovery assay was performed starting 24 h after transfection in serum-free medium C Representative images of scratches taken at time 0, 24 and 48 h in Sk-Mel-28. D Representative images of scratches taken at time 0, 24, and 48 h in Ma-Mel-19. E Quantification of the migratory ability of Sk-Mel-28 cells expressed as the percentage of the migration area evaluated after 24 and 48 h from the scratch formation. F Quantification of the migratory ability of Ma-Mel-19 cells expressed as the percentage of the migration area assessed after 24 and 48 h from the scratch formation. G Biological pathways regulated by all three tested miRNAs. Data are shown as the mean ± SEM of four independent experiments (N = 4). *p < 0.05 by Student’s t-test.

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