Fig. 4: LAL protein content and activity is affected in an in vivo model of NAFLD.
From: Molecular and histological traits of reduced lysosomal acid lipase activity in the fatty liver
C57BL/6J mice were fed for 4 and 8 months with high-fat diet (HFD) or normal diet (ND). A Immunohistochemistry analysis of LAL in liver by using different antibodies recognizing total LAL (C-term) or non-ubiquitinated LAL (N-term) and the relative quantification compared to ND. B Evaluation of total LAL protein in liver homogenates by western blot analysis (left panel) and relative densitometric analysis (right panel). Ponceau red staining was used as loading control. C LAL activity determined by spectrophotometric enzymatic assay. D Analysis of LIPA mRNA levels by qPCR. E–G Analysis of LAL localization by confocal microscopy. The lysosomal marker LAMP1 (red) and total LAL protein (C-term) (green) were shown (E) and their abundance (F) and colocalization (G) were evaluated. Representative immunohistochemistry and immunofluorescent images are reported that are from one mouse out of 3 for each group. Data are expressed as mean ± SD (*p < 0.05; **p < 0.01, ***p < 0.005, vs ND; n = 3 each group). Original magnification X100 (A); X400 (E), high-power field X600 (E). Scale bar 250 μm (A); 25 μm (E).
