Fig. 2: LOXL2 is induced by hypoxia in PDAC.

A LOXL2 mRNA expression in PDAC cells used for LOXL2 overexpression and knockdown exposed to hypoxia for 0–48 h examined by qRT-PCR. B LOXL2 protein expression in PDAC cells used for LOXL2 overexpression or knockdown exposed to hypoxia for 0–48 h examined by western blotting. C, D The expression of LOXL2 and HIF1α at mRNA (C) and protein (D) levels in the indicated cells transiently transfected with siRNAs targeting HIF1α exposed to normoxia or hypoxia for 24 h. E Correlation between LOXL2 expression and the 15-gene hypoxia signature in the PDAC samples of TCGA cohort. F Overall survival and disease-free survival analyses of PDAC patients with different hypoxia status in PDAC samples of TCGA cohort. G Positive correlation between LOXL2 expression and gene set HALLMARK_HYPOXIA in PDAC samples analyzed by GSEA based on the data from GEO and TCGA databases. *p < 0.05, **p < 0.01.