Fig. 5: LOXL2 enhances aerobic glycolysis in PDAC cells. | Cell Death & Disease

Fig. 5: LOXL2 enhances aerobic glycolysis in PDAC cells.

From: Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness

Fig. 5

A Positive correlation between LOXL2 expression and gene set HALLMARK_GLYCOLYSIS in PDAC samples analyzed by GSEA based on the data from GEO and TCGA databases. NES Normalized enrichment score, FDR False discovery rates. B, C Analysis of mRNA levels of glycolytic genes in LOXL2 knockdown and control cells (B) as well as in LOXL2-overexpressing and vector control cells (C) examined by qRT-PCR. D, E Analysis of protein levels of glycolytic genes in LOXL2 knockdown and control cells (D) as well as in LOXL2-overexpressing and vector control cells (E) examined by western blotting. F Glucose uptake and lactate production in LOXL2 knockdown and control cells. G Glucose uptake and lactate production in LOXL2-overexpressing and vector control cells. H Extracellular acid ratio (ECAR) in LOXL2 knockdown and control cells. I ECAR in LOXL2-overexpressing and vector control cells. J Representative IHC images of LOXL2 staining in PDAC tissues with the maximum standardized uptake value (SUVmax) (left panel) and comparison of the SUVmax between LOXL2-high and LOXL2-low patients (right panel). Scale bar: 50 μm. *p < 0.05, **p < 0.01.

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