Fig. 8: The catalytic activity is responsible for the oncogenic roles of LOXL2. | Cell Death & Disease

Fig. 8: The catalytic activity is responsible for the oncogenic roles of LOXL2.

From: Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness

Fig. 8

A Schematic representation of wild-type LOXL2, deletion mutant (ΔLOXL2), and double point mutant (H626Q/H628Q) with the HA-tag. B Western blotting showing the indicated LOXL2 variants stably expressed in PDAC cells using anti-LOXL2 and anti-HA antibodies. C Intracellular hydrogen peroxide measured in PDAC cells stably transfected with the indicated LOXL2 variants. D Western blotting showing hydroxylated HIF1α (HIF1α-OH) expression in PDAC cells stably transfected with the indicated LOXL2 variants treated with 10 μM MG132 for 6 h under normoxic and hypoxic conditions. E Western blotting showing HIF1α expression in PDAC cells stably transfected with the indicated LOXL2 variants under normoxic and hypoxic conditions. F Extracellular acid ratio (ECAR) in PDAC cells stably transfected with the indicated LOXL2 variants. G CCK-8 assay of PDAC cells stably transfected with the indicated LOXL2 variants. H, I Transwell migration (H) and invasion (I) assays of PDAC cells stably transfected with the indicated LOXL2 variants. J Western blotting showing HIF1α expression in LOXL2-overexpressing and vector control cells treated with 10 mM N-acetylcysteine (NAC) for 24 h under normoxic and hypoxic conditions. *p < 0.05, **p < 0.01, ns No significance.

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