Fig. 1: NGF promotes LOX-dependent cell migration and invasion in human chondrosarcoma. | Cell Death & Disease

Fig. 1: NGF promotes LOX-dependent cell migration and invasion in human chondrosarcoma.

From: Nerve growth factor promotes lysyl oxidase-dependent chondrosarcoma cell metastasis by suppressing miR-149-5p synthesis

Fig. 1

A–C Cells were incubated with NGF (30–100 ng/mL) for 18–24 h and cell invasion and migration was examined by the Transwell assay. D JJ012 cells were incubated with NGF (100 ng/mL) for 24 h and qPCR examined the levels of mRNA expression for all LOX family members. E–G Cells were transfected with LOX siRNAs for 24 h, and then stimulated with NGF for 18–24 h. Cell invasion and migration, as well as levels of LOX expression, were examined by Transwell and western blot assays. (H&I) Cells were incubated with NGF (30–100 ng/mL) for 24 h and levels of LOX mRNA and protein expression were examined by qPCR and western blot assays. *p < 0.05 compared with the control group; #p < 0.05 compared with the NGF-treated group.

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