Fig. 2: The PI3K pathway mediates NGF-induced LOX expression and cell motility. | Cell Death & Disease

Fig. 2: The PI3K pathway mediates NGF-induced LOX expression and cell motility.

From: Nerve growth factor promotes lysyl oxidase-dependent chondrosarcoma cell metastasis by suppressing miR-149-5p synthesis

Fig. 2

A Cells were incubated with NGF for the indicated time intervals; p85 phosphorylation was examined by western blot. B–D Cells were treated with PI3K inhibitors (Ly2942002 and wortmannin) for 30 min, and then stimulated with NGF for 18–24 h. Cell migratory and invasive activities, as well as levels of LOX expression, were examined by Transwell and qPCR assays. E Cells were transfected with a p85 siRNA and p85 expression was examined by western blot. F–H Cells were transfected with a p85 siRNA for 24 h, and then stimulated with NGF for 18–24 h. Cell invasion and migration and levels of LOX expression were examined by Transwell and qPCR assays. *p < 0.05 compared with the control group; #p < 0.05 compared with the NGF-treated group.

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