Fig. 3: The Akt pathway mediates NGF-induced LOX expression and cell motility. | Cell Death & Disease

Fig. 3: The Akt pathway mediates NGF-induced LOX expression and cell motility.

From: Nerve growth factor promotes lysyl oxidase-dependent chondrosarcoma cell metastasis by suppressing miR-149-5p synthesis

Fig. 3

A Cells were incubated with NGF for the indicated time intervals; Akt phosphorylation was examined by western blot. B–D Cells were pretreated with a Akt inhibitor (Akt i) for 30 min, and then stimulated with NGF for 18–24 h. Cell invasion and migration and levels of LOX expression were examined by Transwell and qPCR assays. E Cells were transfected with a Akt siRNA and Akt expression was examined by western blot. F–H Cells were transfected with a Akt siRNA for 24 h, and then stimulated with NGF for 18–24 h. Cell migratory and invasive activities, as well as levels of LOX expression, were examined by Transwell and qPCR assays. I Cells were pretreated with PI3K inhibitors for 30 min and then stimulated with NGF for 15 min; Akt phosphorylation was examined by western blot. *p < 0.05 compared with the control group; #p < 0.05 compared with the NGF-treated group.

Back to article page