Fig. 2: GPR120 reduced CD4+ T cell responses in mice with liver injury. | Cell Death & Disease

Fig. 2: GPR120 reduced CD4+ T cell responses in mice with liver injury.

From: GPR120 induces regulatory dendritic cells by inhibiting HK2-dependent glycolysis to alleviate fulminant hepatic failure

Fig. 2

Wild-type (WT) and GPR120−/− (GPR120 KO) mice were injected with P. acnes (P.ac). Vehicle or TUG891 (10 mg/kg) was administered i.p. to WT mice on days 0, 2, 4, and 6 after P. acnes injection. Livers and spleens were isolated on day 7 (n = 7 mice per group). a Absolute numbers of total MNCs in livers and spleens were analyzed by flow cytometry. b Percentages of CD4+ T cells in total MNCs in livers and spleens were analyzed by flow cytometry. c The expression levels of CD44 and CD62L in CD4+ T cells in the livers and spleens were analyzed by flow cytometry. d On day 6 after priming, BrdU was injected i.p. into the mice. MNCs were isolated from livers and spleens the next day. Cells were stained for CD4 and BrdU. The frequencies of CD4+BrdU+ T cells in the livers and spleens were determined by flow cytometry. e The percentages of TNF-α+, IFN-γ+, and Foxp3+ cells in CD4+ T cells were assessed by intracellular staining and flow cytometry. f The levels of serum TNF-α, IFN-γ, IL-4 and IL-10 were measured by enzyme-linked immunosorbent assay. The results are representative of three to six independent experiments and presented as the mean ± SEM. Significant differences were analyzed by One-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ns no significance.

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