Fig. 2: FGF2 and ACLY are two downstream molecules of ONECUT2. | Cell Death & Disease

Fig. 2: FGF2 and ACLY are two downstream molecules of ONECUT2.

From: ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY

Fig. 2

A The human Cancer PathwayFinder RT2 Profiler PCR Array was performed in PLC/PRF/5 and SNU398 cells infected with ONECUT2-overexpressing lentivirus. Venn diagram showing the overlap of upregulated genes in both PLC/PRF/5-ONECUT2 and SNU398-ONECUT2 cells (fold change >2.0). B RT-PCR showing mRNA levels of FGF2 and ACLY in ONECUT2-overexpressing PLC/PRF/5 cells and ONECUT2-silencing MHCC97H cells. C Protein levels of FGF2 and ACLY were measured in ONECUT2-overexpressing PLC/PRF/5 cells and ONECUT2-silencing MHCC97H cells by western blotting. D The level of secreted FGF2 was measured in ONECUT2-overexpressing PLC/PRF/5 cells and ONECUT2-silencing MHCC97H cells by ELISA. E Luciferase reporter assays were performed in PLC/PRF/5 cells after cotransfection with FGF2 or ACLY promoter and pCMV-ONECUT2 plasmid. F–G The relative luciferase activities were measured in PLC/PRF/5 cells after cotransfection with truncations and mutations of FGF2 or ACLY promoter and pCMV-ONECUT2 plasmid. H–I The direct binding of ONECUT2 to the promoters of FGF2 and ACLY was determined in HCC cells and tissues by ChIP assays. Data are mean ± SD. *P < 0.05, **P < 0.01.

Back to article page