Fig. 4: ONECUT2 promotes HCC metastasis by upregulating FGF2 and ACLY. | Cell Death & Disease

Fig. 4: ONECUT2 promotes HCC metastasis by upregulating FGF2 and ACLY.

From: ONECUT2 facilitates hepatocellular carcinoma metastasis by transcriptionally upregulating FGF2 and ACLY

Fig. 4

A Protein levels of ONECUT2, FGF2, and ACLY were measured in PLC/PRF/5 cells cotransfected with ONECUT2-overexpressing lentivirus and FGF2-silencing or ACLY-silencing lentivirus and MHCC97H cells cotransfected with ONECUT2-silencing lentivirus and FGF2-overexpressing or ACLY-overexpressing lentivirus by western blotting. B Knockdown of FGF2 or ACLY was performed in ONECUT2-overexpressing PLC/PRF/5 cells, and overexpression of FGF2 or ACLY was performed in ONECUT2-silencing MHCC97H cells. Next, transwell assays were performed. C–H ONECUT2 facilitated HCC metastasis by inducing FGF2 and ACLY. Representative bioluminescent imaging (C), bioluminescent signals (D), the incidence of pulmonary metastasis (E), number of pulmonary metastasis foci (F), overall survival (G), and H&E staining of the lungs (H) among four groups with ten mice in each group 9 weeks after orthotopic implantation of the indicated cells. Data are mean ± SD. *P < 0.05, **P < 0.01.

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